CRADD Kit ELISA

N° du produit ABIN6951081

Détail du produit CRADD Kit ELISA

Antigène: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human CRADD ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Autre désignation: CRADD (CRADD Produits)

Synonymes: cradd Kit ELISA, MGC89035 Kit ELISA, zgc:101717 Kit ELISA, CRADD Kit ELISA, DKFZp468A2219 Kit ELISA, LOC100226369 Kit ELISA, raidd Kit ELISA, MGC78788 Kit ELISA, MRT34 Kit ELISA, RAIDD Kit ELISA, CASP2 and RIPK1 domain containing adaptor with death domain Kit ELISA, CASP2 and RIPK1 domain containing adaptor with death, gene 1 Kit ELISA, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog Kit ELISA, CRADD Kit ELISA, cradd.1 Kit ELISA, cradd Kit ELISA, cradd.2.S Kit ELISA, Cradd Kit ELISA

ID gène: 8738

UniProt: P78560

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity