RAD51 Kit ELISA

N° du produit ABIN6951284

Détail du produit RAD51 Kit ELISA

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RAD51 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Autre désignation: RAD51 (RAD51 Produits)

Synonymes: BRCC5 Kit ELISA, HRAD51 Kit ELISA, HsRad51 Kit ELISA, HsT16930 Kit ELISA, MRMV2 Kit ELISA, RAD51A Kit ELISA, RECA Kit ELISA, CRAD51 Kit ELISA, wu:fb38e12 Kit ELISA, zgc:77754 Kit ELISA, RGD1563603 Kit ELISA, ATRAD51 Kit ELISA, F22D1.20 Kit ELISA, F22D1_20 Kit ELISA, RAS associated with diabetes protein 51 Kit ELISA, AV304093 Kit ELISA, Rad51a Kit ELISA, Reca Kit ELISA, brcc5 Kit ELISA, rad51 Kit ELISA, rad51a Kit ELISA, reca Kit ELISA, xrad51 Kit ELISA, RAD51 recombinase Kit ELISA, RAS associated with diabetes protein 51 Kit ELISA, RAD51 recombinase L homeolog Kit ELISA, RAD51 Kit ELISA, rad51 Kit ELISA, Rad51 Kit ELISA, rad51.L Kit ELISA

ID gène: 5888

UniProt: Q06609

Pathways: Réparation de l'ADN