TNF alpha Kit ELISA

N° du produit ABIN6974630

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris TNF alpha. Il y a 46 publications disponibles.

Aperçu rapide pour TNF alpha Kit ELISA (ABIN6974630)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Détail du produit TNF alpha Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: For the quantitative determination of mouse tumor necrosis factor α (TNF-α) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of mouse TNF-α. No significant cross-reactivity or interference between mouse TNF-α and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse TNF-α and all the analogues, therefore, cross reaction may still exist. 3

Sensibilité: 3.9 pg/mL

Ingrédients:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Préparation des réactifs:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 1000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (1000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).

9 Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution 5 *Provided this is within the expiration date of the kit.

Date de péremption: 6 months

Références pour TNF alpha Kit ELISA (ABIN6974630)

Chen, Xun, Yang, Ji, Liu, Qi, Wang, Zhang, Shen, Ponte, Mehraj, Routy, Lu: "Plasma Indoleamine 2,3-Dioxygenase Activity Is Associated With the Size of the Human Immunodeficiency Virus Reservoir in Patients Receiving Antiretroviral Therapy." dans: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, Vol. 68, Issue 8, pp. 1274-1281, (2020) (PubMed).

Liu, Zhang, Zheng, Song, Liu, Huang, Bai, Huang, Lin, Zhu, Hu, Mi, Liu: "Activating the pregnane X receptor by imperatorin attenuates dextran sulphate sodium-induced colitis in mice." dans: British journal of pharmacology, Vol. 175, Issue 17, pp. 3563-3580, (2019) (PubMed).

Guo, Sun, Li, Zhang, Bu, Wang, Lai: "Melatonin ameliorates restraint stress-induced oxidative stress and apoptosis in testicular cells via NF-κB/iNOS and Nrf2/ HO-1 signaling pathway." dans: Scientific reports, Vol. 7, Issue 1, pp. 9599, (2019) (PubMed).

Jin, Zheng, Song, Xu, Kou, Zhou, Zhang, Zhu, Wan, Wei, Zhang: "A nano silicon adjuvant enhances inactivated transmissible gastroenteritis vaccine through activation the Toll-like receptors and promotes humoral and cellular immune responses." dans: Nanomedicine : nanotechnology, biology, and medicine, Vol. 14, Issue 4, pp. 1201-1212, (2019) (PubMed).

Chen, Chang, Huang, Xiao, Zhou, Zhang, Li, Zhou, Tang, Lin, Du, Zhang: "Aromatic-Turmerone Attenuates LPS-Induced Neuroinflammation and Consequent Memory Impairment by Targeting TLR4-Dependent Signaling Pathway." dans: Molecular nutrition & food research, Vol. 62, Issue 2, (2018) (PubMed).

Liu, You, Zhang, Ma, Dong, Chen, Jiang, Yun: "The Hsp90 inhibitor 17-DMAG decreases infection of porcine circovirus type 2 in mice." dans: Microbial pathogenesis, Vol. 109, pp. 248-252, (2018) (PubMed).

Xu, Guo, Zhao, He, Wang, Lin, Wang, Liu: "Punicalagin, a PTP1B inhibitor, induces M2c phenotype polarization via up-regulation of HO-1 in murine macrophages." dans: Free radical biology & medicine, Vol. 110, pp. 408-420, (2018) (PubMed).

Zhuang, Shou, Lu, Wang, Qiu, Wang, He, Chen, Jiao, Zhang: "Arachidonic acid sex-dependently affects obesity through linking gut microbiota-driven inflammation to hypothalamus-adipose-liver axis." dans: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1863, Issue 11, pp. 2715-2726, (2018) (PubMed).

Kasimu, Chen, Xie, Li: "Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity." dans: International journal of biological macromolecules, Vol. 105, Issue Pt 1, pp. 452-462, (2018) (PubMed).

Chen, Dou, Luo, Zhang, Li, Zeng, Su, Xie, Lai, Li: "β-Patchoulene from patchouli oil protects against LPS-induced acute lung injury via suppressing NF-κB and activating Nrf2 pathways." dans: International immunopharmacology, Vol. 50, pp. 270-278, (2018) (PubMed).

Wang, Du, Hua, Fu, Niu, Zhang, Zhao, Zhang, Wan: "The EspF N-Terminal of Enterohemorrhagic Escherichia coli O157:H7 EDL933w Imparts Stronger Toxicity Effects on HT-29 Cells than the C-Terminal." dans: Frontiers in cellular and infection microbiology, Vol. 7, pp. 410, (2018) (PubMed).

Xu, Bai, Chen, Li, Qin, Meng, Zhang: "6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice." dans: Biomedicine & pharmacotherapy, Vol. 97, pp. 633-641, (2018) (PubMed).

Xiang, Wang, He, Han, Zhou, Chen, Li, Ma: "The in vitro and in vivo biocompatibility evaluation of electrospun recombinant spider silk protein/PCL/gelatin for small caliber vascular tissue engineering scaffolds." dans: Colloids and surfaces. B, Biointerfaces, Vol. 163, pp. 19-28, (2018) (PubMed).

Dong, Dai, Ding, Wang, Zhang: "Vaccination with FasL-/TCL plus MHSP65 induces improved anti-lung cancer immunity in mice." dans: International immunopharmacology, Vol. 55, pp. 306-311, (2018) (PubMed).

Wen, Mei, Ye, Liu, Xu, Miao, Du, Chen, Li, Li: "Chrysophanol demonstrates anti-inflammatory properties in LPS-primed RAW 264.7 macrophages through activating PPAR-γ." dans: International immunopharmacology, Vol. 56, pp. 90-97, (2018) (PubMed).

Tang, Wu, Lei, Yi, Xu, Lan, Yang, Liu: "HIF1α deletion facilitates adipose stem cells to repair renal fibrosis in diabetic mice." dans: In vitro cellular & developmental biology. Animal, Vol. 54, Issue 4, pp. 272-286, (2018) (PubMed).

Wu, Zhang: "The involvement of Nox4 in fine particulate matter exposure-induced cardiac injury in mice." dans: The Journal of toxicological sciences, Vol. 43, Issue 3, pp. 171-181, (2018) (PubMed).

Liu, Sha, Yang, Zhao, Zhu, Gao, Zhang, Wen: "Impact of Morphology on Iron Oxide Nanoparticles-Induced Inflammasome Activation in Macrophages." dans: ACS applied materials & interfaces, (2018) (PubMed).

Gao, Chen, Peng, Yang, Wang, Lv, Shen: "Caveolin-1 protects against hepatic ischemia/reperfusion injury through ameliorating peroxynitrite-mediated cell death." dans: Free radical biology & medicine, Vol. 95, pp. 209-15, (2017) (PubMed).

Velasco-Ortega, Delgado-Ruiz, López-López: "Dentistry and Diabetes: The Influence of Diabetes in Oral Diseases and Dental Treatments." dans: Journal of diabetes research, Vol. 2016, pp. 6073190, (2017) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet: TNF

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome