C-JUN Kit ELISA

N° du produit ABIN7830499

Kit ELISA C-JUN Humain, Colorimetric test pour la quantification de Humain C-JUN.

Aperçu rapide pour C-JUN Kit ELISA (ABIN7830499)

Antigène: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit C-JUN Kit ELISA

Fonction: JUN (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JUN expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Autre désignation: JUN

Sujet:

Synonyms: AP-1|AP1|c-Jun

Gene Description: jun oncogene

ID gène: 3725

UniProt: P05412

Pathways: Signalisation MAPK, Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins