EPH Receptor B2 Kit ELISA

N° du produit ABIN7830629

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain EPH Receptor B2.

Aperçu rapide pour EPH Receptor B2 Kit ELISA (ABIN7830629)

Antigène: EPH Receptor B2 (EPHB2)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit EPH Receptor B2 Kit ELISA

Fonction: EPHB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHB2 expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: EPH Receptor B2 (EPHB2)

Autre désignation: EPHB2

Sujet:

Synonyms: CAPB|DRT|EPHT3|ERK|Hek5|MGC87492|PCBC|Tyro5

Gene Description: EPH receptor B2

ID gène: 2048

UniProt: P29323

Pathways: Signalisation RTK, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins