LCK Kit ELISA

N° du produit ABIN7830735

Le kit ELISA LCK (ABIN7830735) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain LCK.

Aperçu rapide pour LCK Kit ELISA (ABIN7830735)

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit LCK Kit ELISA

Fonction: LCK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LCK expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Autre désignation: LCK

Sujet:

Synonyms: YT16|p56lck|pp58lck

Gene Description: lymphocyte-specific protein tyrosine kinase

ID gène: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling