MDM2 Kit ELISA

N° du produit ABIN7830754

Kit ELISA MDM2 Humain, Colorimetric test pour la quantification de Humain MDM2.

Aperçu rapide pour MDM2 Kit ELISA (ABIN7830754)

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit MDM2 Kit ELISA

Fonction: MDM2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MDM2 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Autre désignation: MDM2

Sujet:

Synonyms: HDMX|MGC71221|hdm2

Gene Description: Mdm2 p53 binding protein homolog (mouse)

ID gène: 4193

UniProt: Q00987

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway