STAT3 Kit ELISA
Aperçu rapide pour STAT3 Kit ELISA (ABIN7830902)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- STAT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of STAT3 expression in cultured cells.
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Analytical Method
- Qualitative
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Réactivité croisée
- Souris, Rat
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Autre désignation
- STAT3
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Sujet
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Synonyms: APRF|FLJ20882|HIES|MGC16063
Gene Description: signal transducer and activator of transcription 3 (acute-phase response factor)
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ID gène
- 6774
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Pathways
- Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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