MDM2 Kit ELISA

N° du produit ABIN8083417

Kit ELISA MDM2 Humain et Souris, Colorimetric test pour la quantification de Humain et Souris MDM2.

Aperçu rapide pour MDM2 Kit ELISA (ABIN8083417)

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Épitope: pSer166

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Culture Cells

Détail du produit MDM2 Kit ELISA

Fonction: Phospho-MDM2 (Ser166) Colorimetric Cell-Based ELISA Kit for detecting Phospho-MDM2 (Ser166) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Durée du test: 4 - 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 6 months

Détail du antigène

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Autre désignation: MDM2

Sujet: Gene Aliases: Double minute 2 protein, HDM2, Oncoprotein Mdm2, p53-binding protein Mdm2, Ubiquitin-protein ligase E3 Mdm2

ID gène: 4193

UniProt: Q00987

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway