MMP 9 Kit ELISA

N° du produit ABIN955938

Détail du produit MMP 9 Kit ELISA

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of pig MMP9 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of pig MMP9. No significant cross- reactivity or interference was observed.

Sensibilité: The minimum detectable dose of pig MMP9 is typically less than 0.041 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Attributs du produit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP9. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP9 in the samples is then determined by comparing the O.D. of the samples to the calibration curve.

Ingrédients: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Matériel non inclus: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Commentaires:

The calibration curve concentrations used for the ELISA's were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL.

Plaque: Pre-coated

Calcul des résultats:

Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a calibration curve by plotting the mean absorbance for each calibrator on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the MMP9 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Date de péremption: The expiry date is stated on the label.

Détail du antigène

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Autre désignation: MMP9 (MMP9 Produits)

Synonymes: CLG4B Kit ELISA, GELB Kit ELISA, MANDP2 Kit ELISA, MMP-9 Kit ELISA, AW743869 Kit ELISA, B/MMP9 Kit ELISA, Clg4b Kit ELISA, pro-MMP-9 Kit ELISA, mmp-9 Kit ELISA, fgMMP-9 Kit ELISA, mmp9 Kit ELISA, ZFMMP-9 Kit ELISA, wu:fb02g06 Kit ELISA, wu:fb07b05 Kit ELISA, wu:fi98c09 Kit ELISA, wu:fj05a08 Kit ELISA, zgc:64165 Kit ELISA, clg4b Kit ELISA, gelb Kit ELISA, mandp2 Kit ELISA, matrix metallopeptidase 9 Kit ELISA, collagenase Kit ELISA, matrix metalloproteinase 9 Kit ELISA, matrix metallopeptidase 9 S homeolog Kit ELISA, MMP9 Kit ELISA, RB3913 Kit ELISA, BPSS0666 Kit ELISA, Sbal_3732 Kit ELISA, Bcer98_0486 Kit ELISA, Shew185_0630 Kit ELISA, Shal_3056 Kit ELISA, Sbal195_0657 Kit ELISA, Lbys_3550 Kit ELISA, Palpr_2084 Kit ELISA, Mmp9 Kit ELISA, mmp9 Kit ELISA, mmp9.S Kit ELISA

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events