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Duodenum Lysate

WB Whole Tissue Lysate Tissue Lysate

N° du produit ABIN2456254

Fiche de données

Espèce du lysat

Human

Application

Western Blotting (WB)

Attributs du produit

Duodenum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7.9, 1.5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10 % glycerol, 1 % NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % beta-mercaptoethanol.

Source: Human duodenum tissue
Diagnosis: Normal
Tissue or Cell type: Duodenum

Lysate Type

Tissue Lysate

Lysate Fraction

Whole Tissue Lysate
BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain

APLP2 293T Cell Transient Overexpression Lysate(Denatured)
APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain

BAG1 293T Cell Transient Overexpression Lysate(Denatured)
BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain

VNN1 293T Cell Transient Overexpression Lysate(Denatured)
VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain

CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)
CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Humain

Hep2 Whole Cell Lysate
WB Whole Cell Lysate Cell Lysate HepG2 Cells Reactivité: Humain

NIH/3T3 Whole Cell Lysate (PDGF Stimulated)
WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reactivité: Souris

DYKDDDDK Positive Control Lysate
PC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivité: Tag

A431 Whole Cell Lysate (EGF Stimulated)
WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reactivité: Humain

CD177 (human): 293T Lysate
CD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells

Indications d'application

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

2 mg/mL

Buffer

SDS sample buffer containing 5 % beta-mercaptoethanol.

Conseil sur la manipulation

Repeated freezing and thawing is not recommended.

Stock

4 °C/-80 °C

Stockage commentaire

Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.

Date de péremption

6 months