Antibodies that are designed to detect mCherry, a red fluorescent protein commonly used as a fluorescent marker in molecular and cell biology research, are utilized for various purposes. mCherry is often fused to target proteins of interest to study their localization, interactions, and dynamics within cells or organisms. Antibodies specific to mCherry can be used to enhance the visualization and analysis of these tagged proteins. Here are a few applications of such antibodies:
Immunofluorescence and Immunohistochemistry (IF/IHC): Antibodies against mCherry can be used to visualize the subcellular localization of mCherry-tagged proteins.
Western Blotting: Antibodies specific to mCherry aid in detecting and confirming protein expression levels in Western blot assays.
Co-Immunoprecipitation (Co-IP) Studies: Antibodies against mCherry enable the isolation of protein complexes involving mCherry-tagged proteins and their interactors.
Protein-Protein Interaction Studies: Antibodies targeting mCherry assist in pull-down experiments to investigate protein-protein interactions.
Flow Cytometry and Cell Sorting: Antibodies recognizing mCherry are used to identify and isolate cells expressing mCherry-tagged proteins.
Live Cell Imaging: Antibodies against mCherry facilitate real-time visualization of mCherry-tagged protein dynamics in live cells.
Enzyme-Linked Immunosorbent Assay (ELISA): ELISA assays can quantify mCherry-tagged protein levels using specific antibodies.
Subcellular Fractionation Studies: Antibodies against mCherry aid in isolating cellular compartments where the tagged protein is located.
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