anti-Buffalo (Bubalus) Chromogranin A anticorps pour Immunofluorescence

Recommended Chromogranin A Antibody (fourni par: Connectez-vous pour afficher )

Antigène
Chromogranin A (CHGA) Anticorps
  • fj05f09
  • si:dkey-177p2.2
  • wu:fj05f09
  • zgc:101749
  • zgc:56075
  • CHGA
  • ChrA
  • CGA
  • cgA
  • chromogranin A
  • uncharacterized CHGA
  • chga
  • CHGA
  • Chga
Reactivité
Buffalo (Bubalus), Chat, Poulet, Boeuf (Vache), Chien, Canard, Poisson, Cobaye, Hamster, Cheval, Humain, Singe, Souris, Ostrich (Struthio camelus), Porc, Psittacula krameri, Rat (Rattus), Mouton
991
311
253
202
166
117
12
10
5
4
3
2
2
1
1
1
1
1
1
1
1
1
Hôte
Lapin
678
316
21
7
1
1
Clonalité
Polyclonal
Conjugué
Inconjugué
40
35
31
31
27
16
16
16
15
15
15
15
15
15
15
15
15
15
14
14
14
14
14
14
14
14
14
14
14
14
14
14
12
1
1
1
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
674
623
452
397
301
212
203
191
160
95
13
13
9
8
6
3
1
1
1
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
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Images

N° du produit ABIN617895
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
22 ABIN617896 IEM ICC IF IHC Rabbit IgG Connectez-vous pour afficher Polyclonal 36

General

Antigène Chromogranin A (CHGA) Anticorps
Reactivité Buffalo (Bubalus), Chat, Poulet, Boeuf (Vache), Chien, Canard, Poisson, Cobaye, Hamster, Cheval, Humain, Singe, Souris, Ostrich (Struthio camelus), Porc, Psittacula krameri, Rat (Rattus), Mouton
(991), (311), (253), (202), (166), (117), (12), (10), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Hôte Lapin
(678), (316), (21), (7), (1), (1)
Clonalité
Polyclonal
Conjugué Inconjugué
(40), (35), (31), (31), (27), (16), (16), (16), (15), (15), (15), (15), (15), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(674), (623), (452), (397), (301), (212), (203), (191), (160), (95), (13), (13), (9), (8), (6), (3), (1), (1), (1)
Pubmed 174 références disponible
Fournisseur Connectez-vous pour afficher

Détail du produit

Détail du antigène Information d'application Stockage Référence Images
 Réactivité croisée Mammifères
Attributs du produit The antibody has a proven strong staining at a 1/1000 - 1/2000 dilution in rat adrenal medulla using Biotin-Streptavidin/HRP detection method.
Purification Whole Serum
Immunogène SP-1 Chromogranin A
Isotype IgG

Détail du antigène

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Antigène
Autre désignation Chromogranin A (CHGA Antibody Extrait)
Sujet Other Names:
Pituitary secretory protein I, CgA, chromogranin A (parathyroid secretory protein 1)
ID gène 281070
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Information d'application

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Restrictions For Research Use only

Stockage

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Format Liquid
Buffer Contains ≤ 0.09 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

Référence

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Produit citée dans:

Leblanc, Langlois, Coulombe, Vaillancourt-Lavigueur, Jones, Carrier, Boudreau, Rivard: "Epithelial Src homology region 2 domain-containing phosphatase-1 restrains intestinal growth, secretory cell differentiation, and tumorigenesis." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, pp. 3512-3526, 2017

Wang, Kwon, Singh, Islam, Liu, Sridhar, Hofmann, Browning: "Type 2 cGMP-dependent protein kinase regulates homeostasis by blocking c-Jun N-terminal kinase in the colon epithelium." dans: Cell death and differentiation, Vol. 21, Issue 3, pp. 427-37, 2014

Reilly, Teo, Low, Amoyo-Brion, Dominguez-Brauer, Elia, Berger, Greicius, Pettersson, Mak: "Lipocalin 2 performs contrasting, location-dependent roles in APCmin tumor initiation and progression." dans: Oncogene, Vol. 32, Issue 10, pp. 1233-9, 2013

Wolfs, Kallapur, Knox, Thuijls, Nitsos, Polglase, Collins, Kroon, Spierings, Shroyer, Newnham, Jobe, Kramer: "Antenatal ureaplasma infection impairs development of the fetal ovine gut in an IL-1-dependent manner." dans: Mucosal immunology, Vol. 6, Issue 3, pp. 547-56, 2013

Rychel, Worley, Hardy, Webb: "Prolonged survival in an aged Labrador retriever with a metastatic insulinoma." dans: Journal of the American Animal Hospital Association, Vol. 49, Issue 3, pp. 224-9, 2013

Ho, Sinha, Verzi, Bernt, Armstrong, Shivdasani: "DOT1L-mediated H3K79 methylation in chromatin is dispensable for Wnt pathway-specific and other intestinal epithelial functions." dans: Molecular and cellular biology, Vol. 33, Issue 9, pp. 1735-45, 2013

Gozalo, Zerfas, Starost, Lambert, Elkins: "Pancreatic endocrine tumour with disseminated pulmonary thromboembolism in an owl monkey (Aotus nancymae)." dans: Journal of comparative pathology, Vol. 149, Issue 1, pp. 132-6, 2013

Gracz, Fuller, Wang, Li, Stelzner, Dunn, Martin, Magness: "Brief report: CD24 and CD44 mark human intestinal epithelial cell populations with characteristics of active and facultative stem cells." dans: Stem cells (Dayton, Ohio), Vol. 31, Issue 9, pp. 2024-30, 2013

Dipaola, Shivakumar, Pfister, Walters, Sabla, Bezerra: "Identification of intramural epithelial networks linked to peribiliary glands that express progenitor cell markers and proliferate after injury in mice." dans: Hepatology (Baltimore, Md.), Vol. 58, Issue 4, pp. 1486-96, 2013

Feng, Sentani, Wiese, Sands, Green, Bommer, Cho, Fearon: "Sox9 induction, ectopic Paneth cells, and mitotic spindle axis defects in mouse colon adenomatous epithelium arising from conditional biallelic Apc inactivation." dans: The American journal of pathology, Vol. 183, Issue 2, pp. 493-503, 2013

Schaffer, Weishaar, Han: "Endobronchial metastasis in a dog with sarcoma." dans: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 25, Issue 4, pp. 546-50, 2013

Hu He, Juhl, Karadimos, El Khattabi, Fitzpatrick, Bonner-Weir, Sharma: "Differentiation of pancreatic endocrine progenitors reversibly blocked by premature induction of MafA." dans: Developmental biology, Vol. 385, Issue 1, pp. 2-12, 2013

Grozinsky-Glasberg, Thomas, Strosberg, Pape, Felder, Tsolakis, Alexandraki, Fraenkel, Saiegh, Reissman, Kaltsas, Gross: "Metastatic type 1 gastric carcinoid: a real threat or just a myth?" dans: World journal of gastroenterology : WJG, Vol. 19, Issue 46, pp. 8687-95, 2013

Gonzalez, Williamson, Piedrahita, Blikslager, Magness: "Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration." dans: PLoS ONE, Vol. 8, Issue 6, pp. e66465, 2013

Roy, Langlois, Carrier, Boudreau, Rivard, Perreault: "Dual regulatory role for phosphatase and tensin homolog in specification of intestinal endocrine cell subtypes." dans: World journal of gastroenterology : WJG, Vol. 18, Issue 14, pp. 1579-89, 2012

Wang, Kwon, Thangaraju, Singh, Liu, Jay, Hofmann, Ganapathy, Browning: "Type 2 cGMP-dependent protein kinase regulates proliferation and differentiation in the colonic mucosa." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 303, Issue 2, pp. G209-19, 2012

Erlandsen, Qvigstad, Fossmark, Bakke, Chen, Sandvik: "Regulated endocrine-specific protein 18 (RESP18) is localized to and regulated in A-like cells and G-cells in rat stomach." dans: Regulatory peptides, Vol. 177, Issue 1-3, pp. 53-9, 2012

Stauffer, Orentas, Lincoln, Khan, Salcedo, Hixon, Back, Wei, Patidar, Song, Hurd, Tsokos, Lai, Eisenhofer, Weiss, Khan, Wigginton: "High-throughput molecular and histopathologic profiling of tumor tissue in a novel transplantable model of murine neuroblastoma: new tools for pediatric drug discovery." dans: Cancer investigation, Vol. 30, Issue 5, pp. 343-63, 2012

Li, Kapoor, Giel-Moloney, Rindi, Leiter: "Notch signaling differentially regulates the cell fate of early endocrine precursor cells and their maturing descendants in the mouse pancreas and intestine." dans: Developmental biology, Vol. 371, Issue 2, pp. 156-69, 2012

Lu, Germano, Ohning, Vu, Pisegna: "PAC1 deficiency in a murine model induces gastric mucosa hypertrophy and higher basal gastric acid output." dans: Journal of molecular neuroscience : MN, Vol. 43, Issue 1, pp. 76-84, 2011

Images

Détail du produit Détail du antigène Information d'application Stockage Référence Haut de la page
Images (Fournisseur)
Immunocytochemistry (ICC) image for anti-Chromogranin A (CHGA) antibody (ABIN617895) Immunocytochemistry (ICC) image for anti-Chromogranin A (CHGA) antibody
'Independent Validation' signe
Antigène CHGA
Numéro du lot 1436001
Application validée Immunohistochemistry
Contrôle positif Macaque hippocampus sections
Contrôle négative No primary antibody
Conclusion

Passed. ABIN617895 specifically labels the targeted antigen in macaque hippocampus in IHC. No signal was detected in sample negative control tissue and in the secondary antibody only control.

Anticorps primaire ABIN617895
Anticorps secondaire goat anti-rabbit IgG, biotin-conjugated antibody (Vector, BA-1000, lot W1002)
Protocole
  • Intracardially perfuse the animal with the fixative solution (4% (w/v) PFA, 15% v/v picric acid saturated solution). Remove the brain and postfixate in the same solution ON at RT.
  • Cut slices (70µm of thickness) in a vibratome.
  • Proceed with a free-floating IHC in a 24-well plate (1 slice, 250µl of volume per well).
  • Wash slices for 10min with 0.1M PBS pH7.4 at RT.
  • Permeabilize slices 3x for 5min with 0.1M PBS containing 1% v/v triton x-100 (PBST).
  • Block non-specific binding with staining solution (PBST containing 10% v/v fetal bovine serum) for 1h at RT.
  • Incubate slices with primary rabbit anti-Chromogranin A (CHGA) antibody (antibodies-online, ABIN617895, lot 1436001) diluted 1:1000 in staining solution ON at RT.
  • Wash slices 3x for 5min with PBST.
  • Incubate slices with secondary goat anti-rabbit IgG, biotin-conjugated antibody (Vector, BA-1000, lot W1002) diluted 1:200 in staining solution for 2h at RT.
  • Wash slices 3x for 5min with PBS.
  • Incubate slices with ABC kit (Vectastain, PK-6100) diluted 1:100 in staining solution for 1h at RT.
  • Wash slices 3x for 5min with PBS.
  • Incubate with 0.05% DAB (Sigma, D5637) and 0.01% H2O2 in PBS, watching reaction at the microscope.
  • Wash slices 3x for 5min with PBS.
  • Mount slices on gelatinated glass slides, dehydrate and coverslip with DPX mountant (VWR, 360294H).
  • Image acquisition with Leica SP-5 confocal microscope, 10x and 20x, resolution 1368x1024.
Notes
  • In macaque sections the labeling obtained with ABIN617895 was the same as previously described in human hippocampus (Munoz (1990)).

  • In mouse hippocampus, however, staining was only observed in unexpected scattered cells, probably interneurons. In rat hippocampus, it was not detected any clear labeling. Further tests are needed to determined if the antibody is not properly detecting murine CHGA or if the antigen is not present in the hippocampus in these two species.

Validation Images
Immunohistochemistry validation image for anti-Chromogranin A (CHGA) antibody (ABIN617895) The CHGA antibody ABIN617895 stained CA2 pyramidal cell bodies and mossy fibers in ma...