This is a synthetic peptide designed for use in combination with anti-KLHL12 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
Concentration
1 mg/mL
Buffer
Final peptide concentration is 1 mg/mL in PBS.
Conseil sur la manipulation
Avoid repeat freeze-thaw cycles.
Stock
-20 °C
Stockage commentaire
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Antigène
KLHL12
(Kelch-Like 12 (Drosophila) (KLHL12))
Synonymes
zgc:92570 Peptide, wu:fi60g03 Peptide, C3ip1 Peptide, C3IP1 Peptide, DKIR Peptide, hDKIR Peptide, Ski2 like RNA helicase Peptide, kelch like family member 12 L homeolog Peptide, kelch like family member 12 Peptide, kelch-like family member 12 Peptide, kelch-like 12 Peptide, SKIV2L Peptide, klhl12.L Peptide, KLHL12 Peptide, klhl12 Peptide, Klhl12 Peptide
Sujet
KLHL12 is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.