GEM Antibody ships at 0.5 mg/mL and the GEM Peptide ships as a 100 μ,g pellet. When peptide is reconstituted in 200 μ,L water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
GEM Peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
Stock
-20 °C
Stockage commentaire
Store at -20°C. When reconstituted minimize freezing and thawing.
Antigène
GEM
(GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))
Synonymes
KIR Peptide, GEM Peptide, DKFZp470D0612 Peptide, LOC100221129 Peptide, kir Peptide, xgem Peptide, zgc:153003 Peptide, Gem Peptide, AV020497 Peptide, GTP binding protein overexpressed in skeletal muscle Peptide, geminin, DNA replication inhibitor Peptide, GTP binding protein (gene overexpressed in skeletal muscle) Peptide, GEM Peptide, gem Peptide, Gem Peptide, GMNN Peptide