Semaphorin 3E Peptide is used for blocking the activity of Semaphorin 3E antibody . The Semaphorin 3E antibody ships at 0.5 mg/mL and the Semaphorin 3E Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Semaphorin 3E peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
Stock
-20 °C
Stockage commentaire
Store at -20°C. When reconstituted minimize freezing and thawing.
Antigène
SEMA3E
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))