TIM Protein (AA 1-249) (His tag)

N° du produit ABIN1097652

Détail du produit

Antigène: TIM (Triosephosphate Isomerase (TIM))

Type de proteíne: Recombinant

Attributs du protein: AA 1-249

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette TIM protéine est marqué à la His tag.

Fonction: Recombinant Human Triosephosphate Isomerase/TIM (N-6His)

Séquence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

Attributs du produit: Recombinant Human Triosephosphate Isomerase/TIM is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln249) of Human TPI1 fused with a His tag at the N-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 8.0.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: TIM (Triosephosphate Isomerase (TIM))

Autre désignation: Triosephosphate Isomerase/TIM (TIM Produits)

Synonymes: F26H11.7 Protein, F26H11_7 Protein, PDTPI Protein, PLASTID ISOFORM TRIOSE PHOSPHATE ISOMERASE Protein, TRIOSEPHOSPHATE ISOMERASE Protein, triosephosphate isomerase Protein, triosephosphate isomerase Protein, TIM Protein

Sub Type: Fusionprotein

Sujet: Triose-phosphate isomerase, also named Triose-phosphate isomerase, TPI and TIM, is an enzyme that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. TPI has been found in nearly every organism searched for the enzyme, including animals such as mammals and insects as well as in fungi, plants, and bacteria. However, some bacteria that do not perform glycolysis, like ureaplasmas, lack TPI. TPI plays an important role in glycolysis and is essential for efficient energy production. TPI deficiency is an autosomal recessive disorder and the most severe clinical disorder of glycolysis. Triose phosphate isomerase deficiency is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection and characterized by chronic hemolytic anemia.
Alternative Names: Triosephosphate Isomerase, TIM, Triose-Phosphate Isomerase, TPI1, TPI

Poids moléculaire: 28.8 kDa

UniProt: P60174