Ube2t Protein (AA 1-197) (His tag)

N° du produit ABIN1097739

Détail du produit

Antigène: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Type de proteíne: Recombinant

Attributs du protein: AA 1-197

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Ube2t protéine est marqué à la His tag.

Fonction: Recombinant Human Ubiquitin-Conjugating Enzyme E2 T/UBE2T (N-6His)

Séquence: MGSSHHHHHH SSGLVPRGSH MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV

Attributs du produit: Recombinant Human Ubiquitin-Conjugating Enzyme E2 T/UBE2T is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val197) of Human UBE2T.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Autre désignation: Ubiquitin-Conjugating Enzyme E2 T/UBE2T (Ube2t Produits)

Synonymes: PIG50 Protein, UBE2T Protein, 2700084L22Rik Protein, C80607 Protein, RGD1310816 Protein, hspc150 Protein, wu:fj86h11 Protein, zgc:153687 Protein, ubiquitin conjugating enzyme E2 T Protein, ubiquitin-conjugating enzyme E2T Protein, ubiquitin conjugating enzyme E2T S homeolog Protein, ubiquitin-conjugating enzyme E2T (putative) Protein, UBE2T Protein, Ube2t Protein, ube2t.S Protein, ube2t Protein

Sujet: Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 T, Cell Proliferation-Inducing Gene 50 Protein, Ubiquitin Carrier Protein T, Ubiquitin-Protein Ligase T, UBE2T

Poids moléculaire: 24.7 kDa

UniProt: Q9NPD8

Pathways: Chromatin Binding