GTF2E2 Protein (His tag)

N° du produit ABIN1098619

Détail du produit

Antigène: GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GTF2E2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Purification: purified by using conventional chromatography.

Pureté: > 90 % by SDS - PAGE

Information d'application

Commentaires:

Synonyms: Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1 M NaCl, 1 MM DTT

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))

Autre désignation: GTF2E2 (GTF2E2 Produits)

Synonymes: 34kDa Protein, AI462509 Protein, C330006J08Rik Protein, wu:fa05d01 Protein, zgc:77074 Protein, FE Protein, TF2E2 Protein, TFIIE-B Protein, general transcription factor II E, polypeptide 2 (beta subunit) Protein, general transcription factor IIE subunit 2 L homeolog Protein, general transcription factor IIE subunit 2 Protein, general transcription factor IIE, polypeptide 2, beta Protein, Gtf2e2 Protein, gtf2e2.L Protein, gtf2e2 Protein, GTF2E2 Protein

Sujet: GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-alpha) and two of 34,000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 35.6 kDa (315aa), confirmed by MALDI-TOF

NCBI Accession: NP_002086