CPSF4 Protein (His tag)

N° du produit ABIN1098798

Détail du produit

Antigène: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CPSF4 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Pureté: > 85 % by SDS - PAGE

Information d'application

Commentaires:

Synonyms: cleavage and polyadenylation specific factor 4, 30kDa, CPSF30, NAR, NEB1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M UREA, 10% glycerol

Stock: 4 °C

Stockage commentaire: Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Autre désignation: CPSF4 (CPSF4 Produits)

Synonymes: cpsf30 Protein, nar Protein, neb1 Protein, 30kDa Protein, C79664 Protein, CPSF30 Protein, NAR Protein, NEB1 Protein, cleavage and polyadenylation specific factor 4 Protein, cleavage and polyadenylation specific factor 4, 30kDa Protein, cleavage and polyadenylation specific factor 4 S homeolog Protein, CPSF4 Protein, Cpsf4 Protein, LOC733099 Protein, LOC100360200 Protein, cpsf4.S Protein, cpsf4 Protein

Sujet: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Poids moléculaire: 29.9 kDa (267aa)

NCBI Accession: NP_001075028