TGFB2 Protein (His tag)

N° du produit ABIN1112028

Détail du produit

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Type de proteíne: Recombinant

Origine: Humain

Source: Tobacco (Nicotiana benthamiana)

Purification/Conjugué: Cette TGFB2 protéine est marqué à la His tag.

Application: Western Blotting (WB), Immunogen (Imm), ELISA, Standard (STD)

Séquence: HHHHHHALDA AYCFRNVQDN CCLRPLYIDF KRDLGWKWIH EPKGYNANFC AGACPYLWSS DTQHSRVLSL YNTINPEASA SPCCVSQDLE PLTILYYIGK TPKIEQLSNM IVKSCKCS

Specificité: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.

Attributs du produit: Recombinant human TGF beta 2 contains a 6-His tag at the N-terminal end, is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal free product.
Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.
Molecular Formula: C602H909N167O171S10
Isoelectric Point: 7,72
Extinction Coefficient: E 0.1 % (1g/L) = 2.02 (A 280 nm)
This product contains no animal-derived components or impurities. It is produced by transient expression of TGF-beta3 in non-transgenic plants.

Purification: Recombinant human TGF-beta3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Pureté: > 97 % by SDS-PAGE gel

niveau d'endotoxine: < 0.04 EU/µg protein (LAL method)

Information d'application

Commentaires:

Human recombinant protein expressed in Nicotiana benthamiana.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Due to the protein nature, dimmers and multimers may be observed. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.

Concentration: 50 ng/µL

Buffer: Tris HCl 0.05 M buffer at pH 7.4.

Conseil sur la manipulation: Reconstituted protein should be stored in working aliquots at -20 °C and it is recommended to add a carrier protein (0.1 % HSA or BSA). Repeated freezing and thawing is not recommended.

Stock: 4 °C

Référence

Zou, Sun: "An improved recombinant mammalian cell expression system for human transforming growth factor-beta2 and -beta3 preparations." dans: Protein expression and purification, Vol. 50, Issue 1, pp. 9-17, (2006) (PubMed).

Massagué: "The transforming growth factor-beta family." dans: Annual review of cell biology, Vol. 6, pp. 597-641, (1991) (PubMed).

Miller, Pelton, Derynck, Moses: "Transforming growth factor-beta. A family of growth regulatory peptides." dans: Annals of the New York Academy of Sciences, Vol. 593, pp. 208-17, (1990) (PubMed).

ten Dijke, Hansen, Iwata, Pieler, Foulkes: "Identification of another member of the transforming growth factor type beta gene family." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 13, pp. 4715-9, (1988) (PubMed).

Détail du antigène

Antigène: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Autre désignation: TGF beta 2 (TGFB2 Produits)

Synonymes: LDS4 Protein, TGF-beta2 Protein, tgf-beta2 Protein, tgfb2-A Protein, BB105277 Protein, Tgf-beta2 Protein, Tgfb-2 Protein, TGF-beta 2 Protein, Tgfbr2T Protein, TGFB2 Protein, TGFbeta2 Protein, MGF Protein, TGF-B2 Protein, transforming growth factor beta 2 Protein, transforming growth factor beta 2 L homeolog Protein, transforming growth factor, beta 2 Protein, transforming growth factor, beta receptor 2 Protein, TGFB2 Protein, tgfb2.L Protein, Tgfb2 Protein, Tgfbr2 Protein, tgfb2 Protein

Sujet: Synonyms: Transforming growth factor beta-2, TGF-beta-2
Recombinant human TGF-beta2 is a 27.08 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulphide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2 and TGF-beta3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.

Poids moléculaire: 27.08 kDa

ID gène: 7042, 190220

UniProt: P61812

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus