IL12 Protéine
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- Antigène Voir toutes IL12 Protéines
- IL12 (Interleukin 12 (IL12))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Application
- ELISA, Functional Studies (Func)
- Marque
- BD Pharmingen™
- Specificité
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Biological Activity Range: Measured by 2D6 cell proliferation Specific Activity: 0.2 - 2.0 x 10^9 Units /mg
ED50: 5 - 50 pg/ml
Observed dose response: > 200 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation - Attributs du produit
- The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 µg BSA to 1 µg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law.
- Purification
- Recombinant mouse IL-12 p70 is provided lyophilized, containing bovine serum albumin and no preservatives. Recombinant mouse IL-12 p70 was found to be ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.
- Pureté
- ≥ 95 %
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- The endotoxin level is ≤ 0.1 ng per µg of mouse IL-12, as measured in a chromogenic LAL assay.
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- Discover our top product IL12 Protéine
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- Indications d'application
- ELISA Standard: This mouse IL-12 is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA with the purified 9A5 antibody (Cat. No. 554658) as a capture antibody and the biotinylated C17.8 (Cat. No. 554476) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-12 standard from 4000-30 pg/ml should be included in each ELISA plate.
- Commentaires
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BD Pharmingen™ Recombinant Mouse IL-12 p70 - Purified - Clone NA - Reactivity Ms
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized in an aqueous buffered solution containing BSA.
- Conseil sur la manipulation
- Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.
- Stock
- -80 °C
- Stockage commentaire
- Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Cloning and expression of murine IL-12." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).
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Cloning and expression of murine IL-12." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).
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- Antigène
- IL12 (Interleukin 12 (IL12))
- Autre désignation
- IL-12 (p70) (IL12 Produits)
- Synonymes
- CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein
- Sujet
- Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.
- Pathways
- Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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