LIF Protein (AA 23-202)

N° du produit ABIN1344370

Détail du produit

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-202

Origine: Humain, Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Specificité: Mouse and human embryonic stem cells.

Réactivité croisée: Humain, Souris

Attributs du produit: Human LIF (aa 23-202) is fused at the N-terminus to a tag.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.05EU/μg purified protein (LAL test, Lonza).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF (LIF Produits)

Synonymes: CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein

Sujet: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Poids moléculaire: ~46kDa (SDS-PAGE)

UniProt: P15018

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding