Histone H3.3 Protein (AA 2-136, full length) (GST tag)

N° du produit ABIN1607971

Détail du produit

Antigène: Histone H3.3

Type de proteíne: Recombinant

Attributs du protein: AA 2-136, full length

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Histone H3.3 protéine est marqué à la GST tag.

Application: ELISA

Séquence: ARTKQTARKS TGGKAPRKQL ATKAARKSAP STGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSAAIG ALQEASEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA

Attributs du produit: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Pureté: 95 %

Information d'application

Commentaires:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Conseil sur la manipulation: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Stock: -20 °C

Stockage commentaire: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Détail du antigène

Antigène: Histone H3.3

Autre désignation: Histone H3.3 protein (Histone H3.3 Produits)

Synonymes: H3.3A Protein, H3F3 Protein, H3-IX Protein, H3-X Protein, H3.3B Protein, H3F3C Protein, H3F3B Protein, H3 histone family member 3A Protein, H3 histone, family 3A Protein, H3 histone, family 3B Protein, H3 histone, family 3C L homeolog Protein, histone H3.3 Protein, Histone H3.3 Protein, histone H3.3B-like Protein, H3F3A Protein, H3F3B Protein, H3f3a Protein, h3f3c.L Protein, LOC5567559 Protein, Bm1_29875 Protein, Bm1_40030 Protein, PTRG_03457 Protein, Phum_PHUM492720 Protein, UREG_07214 Protein, PAAG_07099 Protein, PITG_06950 Protein, PITG_06953 Protein, PITG_06955 Protein, LOC100361558 Protein, LOC100521680 Protein

Sujet: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Poids moléculaire: 42.6 kD

UniProt: P84243