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Histone H3.3 Protein (His tag)

Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
N° du produit ABIN2722821
  • Antigène Voir toutes Histone H3.3 Protéines
    Histone H3.3
    Type de proteíne
    Recombinant
    Origine
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 6
    • 4
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette Histone H3.3 protéine est marqué à la His tag.
    Application
    Standard (STD), Antibody Production (AbP)
    Attributs du produit
    • Recombinant human Histone H3.3 (full length, N-term HIS tag) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    Pureté
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product Histone H3.3 Protéine
  • Indications d'application
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Commentaires

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 100 mM Arg. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Antigène
    Histone H3.3
    Abstract
    Histone H3.3 Produits
    Synonymes
    H3.3A Protein, H3F3 Protein, H3-IX Protein, H3-X Protein, H3.3B Protein, H3F3C Protein, H3F3B Protein, H3 histone family member 3A Protein, H3 histone, family 3A Protein, H3 histone, family 3B Protein, H3 histone, family 3C L homeolog Protein, histone H3.3 Protein, Histone H3.3 Protein, histone H3.3B-like Protein, H3F3A Protein, H3F3B Protein, H3f3a Protein, h3f3c.L Protein, LOC5567559 Protein, Bm1_29875 Protein, Bm1_40030 Protein, PTRG_03457 Protein, Phum_PHUM492720 Protein, UREG_07214 Protein, PAAG_07099 Protein, PITG_06950 Protein, PITG_06953 Protein, PITG_06955 Protein, LOC100361558 Protein, LOC100521680 Protein
    Sujet
    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
    Poids moléculaire
    15.3 kDa
    NCBI Accession
    NP_005315
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