RNF146 Protein (GST tag)

N° du produit ABIN1741735

Détail du produit

Antigène: RNF146 (Ring Finger Protein 146 (RNF146))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette RNF146 protéine est marqué à la GST tag.

Application: Pull-Down Assay (Pull-Down)

Specificité: WWE Negative Control Resin, ABIN1741735 is identical to the ABIN1741734 resin except for R163A substitution, which effectively abolishes PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
WWE Neg Control Resin, 0.5 mL (0.5 mg fusion protein) supplied as a slurry containing approx. 50 µL resin.

Attributs du produit: WWE Affinity Resin (ABIN1741734) and Negative Control Resin (ABIN1741735) are designed for the isolation and study of poly-ADP-ribosylated (PARylated) proteins. Through the use of this highly specific PAR affinity resin, PARylated proteins are isolated from cell or tissue lysates without the use of anti-PAR antibodies.
RNF146-175

Purification: Affinity chromatography

Pureté: > 95 %

Information d'application

Indications d'application: 20 µL=20 µg per reaction

Commentaires:

70.00

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, and 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze!

Stock: 4 °C

Stockage commentaire: Stable for 6 months from date of shipment when stored at 4 ˚C.

Référence

Zhong, Yeh, Hao, Pourtabatabaei, Mahata, Shao, Chessler, Chi: "Nutritional energy stimulates NAD+ production to promote tankyrase-mediated PARsylation in insulinoma cells." dans: PLoS ONE, Vol. 10, Issue 4, pp. e0122948, (2015) (PubMed).

Détail du antigène

Antigène: RNF146 (Ring Finger Protein 146 (RNF146))

Autre désignation: RNF146 (RNF146 Produits)

Synonymes: RP3-351K20.1 Protein, dJ351K20.1 Protein, 2610509H23Rik Protein, Iduna Protein, RNF146 Protein, rnf146 Protein, dactylidin Protein, wu:fi29e08 Protein, zgc:55909 Protein, ring finger protein 146 Protein, RING finger protein 146 Protein, ring finger protein 146 S homeolog Protein, RNF146 Protein, Rnf146 Protein, rn146 Protein, rnf146.S Protein, rnf146 Protein

Sujet: RNF146 (Iduna) is a RING-domain E3 ubiquitin ligase that positively regulates Wnt signalling. RNF146 directly interacts with poly(ADP-ribose) through its WWE domain. The WWE domain is a conserved globular domain found in multiple PARPs and E3 ligases.

Poids moléculaire: 8 kDa + GST