CPE Protein (AA 42-453) (His tag)

N° du produit ABIN1880662

Détail du produit

Antigène: CPE (Carboxypeptidase E (CPE))

Type de proteíne: Recombinant

Attributs du protein: AA 42-453

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette CPE protéine est marqué à la His tag.

Fonction: Recombinant Human Carboxypeptidase E/CPE (C-6His)

Séquence: RLQQEDGISF EYHRYPELRE ALVSVWLQCT AISRIYTVGR SFEGRELLVI ELSDNPGVHE PGEPEFKYIG NMHGNEAVGR ELLIFLAQYL CNEYQKGNET IVNLIHSTRI HIMPSLNPDG FEKAASQPGE LKDWFVGRSN AQGIDLNRNF PDLDRIVYVN EKEGGPNNHL LKNMKKIVDQ NTKLAPETKA VIHWIMDIPF VLSANLHGGD LVANYPYDET RSGSAHEYSS SPDDAIFQSL ARAYSSFNPA MSDPNRPPCR KNDDDSSFVD GTTNGGAWYS VPGGMQDFNY LSSNCFEITV ELSCEKFPPE ETLKTYWEDN KNSLISYLEQ IHRGVKGFVR DLQGNPIANA TISVEGIDHD VTSAKDGDYW RLLIPGNYKL TASAPGYLAI TKKVAVPYSP AAGVDFELES FSVDHHHHHH

Attributs du produit: Recombinant Human Carboxypeptidase E/CPE is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg42-Ser453) of Human CPE fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM ZnCl2,10 % Glycerol, pH 7.5.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CPE (Carboxypeptidase E (CPE))

Autre désignation: CPE (CPE Produits)

Synonymes: CPE Protein, CPH Protein, Cph-1 Protein, Cph1 Protein, R74677 Protein, fat Protein, CARBE Protein, Cph Protein, si:ch211-198g9.2 Protein, zgc:85981 Protein, carboxypeptidase E Protein, carboxypeptidase E L homeolog Protein, CPE Protein, cpe Protein, Cpe Protein, cpe.L Protein

Sub Type: Fusionprotein

Sujet: The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
Alternative Names: Carboxypeptidase E(CPE for short), also called Carboxypeptidase H, Enkephalin convertase, Prohormone-processing carboxypeptidase, is a peripheral membrane protein and a zinc metallo carboxypeptidase which belongs to the peptidase M14 family.

Poids moléculaire: 47.1 kDa

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane