AA 1-305 (Lys82Asn), expressed with a C-terminal TAT peptide.
Pureté
> 95 % by SDS-PAGE and HPLC analyses.
Stérilité
Sterile filtered
niveau d'endotoxine
< 0.2 EU/μg, determined by LAL method.
Restrictions
For Research Use only
Format
Liquid
Buffer
Sterile Filtered solution contains 10 mM PB, 300 mM NaCl, pH 7.4.
Conseil sur la manipulation
Avoid repeated freezing and thawing cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4 °C from date of receipt. For long term storage, aliquot and store at -20 °C or -80 °C.
Antigène
Nanog-TAT
Sujet
NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E. coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2 kDa analyzed by reducing SDS-PAGE. Synonyms: None