AFT1 Protein (AA 1-271) (His tag)

N° du produit ABIN2115465

Détail du produit

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-271

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette AFT1 protéine est marqué à la His tag.

Fonction: Recombinant Human Activating Transcription Factor 1/ATF1 (C-6His)

Séquence: MEDSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV TLTSQTTKTD DPQLKREIRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK TLKDLYSNKS VVEHHHHHH

Attributs du produit: Recombinant Human Cyclic AMP-dependent transcription factor ATF-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val271) of Human ATF1 fused with a 6His tag at the C-terminus..

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Autre désignation: ATF1 (AFT1 Produits)

Synonymes: ews-atf1 Protein, fus/atf-1 Protein, treb36 Protein, ATF1 Protein, EWS-ATF1 Protein, FUS/ATF-1 Protein, TREB36 Protein, Atf-1 Protein, wu:fc92c07 Protein, zgc:64099 Protein, activating transcription factor 1 Protein, activating transcription factor 1 L homeolog Protein, atf1 Protein, ATF1 Protein, MGC85066 Protein, atf1.L Protein, Atf1 Protein

Sujet: Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
Synonyms: Cyclic AMP-dependent transcription factor ATF-1,cAMP-dependent transcription factor ATF-1,Activating transcription factor 1,

Poids moléculaire: 30.3 kDa

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades