LCP2 Protein (AA 1-533) (His tag,T7 tag)

N° du produit ABIN2115473

Détail du produit

Antigène: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-533

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette LCP2 protéine est marqué à la His tag,T7 tag.

Fonction: Recombinant Human Lymphocyte Cytosolic Protein 2/LCP2 (C-6His, N-T7 tag)

Séquence: MASMTGGQQM GRGSMALRNV PFRSEVLGWD PDSLADYFKK LNYKDCEKAV KKYHIDGARF LNLTENDIQK FPKLRVPILS KLSQEINKNE ERRSIFTRKP QVPRFPEETE SHEEDNGGWS SFEEDDYESP NDDQDGEDDG DYESPNEEEE APVEDDADYE PPPSNDEEAL QNSILPAKPF PNSNSMYIDR PPSGKTPQQP PVPPQRPMAA LPPPPAGRNH SPLPPPQTNH EEPSRSRNHK TAKLPAPSID RSTKPPLDRS LAPFDREPFT LGKKPPFSDK PSIPAGRSLG EHLPKIQKPP LPPTTERHER SSPLPGKKPP VPKHGWGPDR RENDEDDVHQ RPLPQPALLP MSSNTFPSRS TKPSPMNPLP SSHMPGAFSE SNSSFPQSAS LPPYFSQGPS NRPPIRAEGR NFPLPLPNKP RPPSPAEEEN SLNEEWYVSY ITRPEAEAAL RKINQDGTFL VRDSSKKTTT NPYVLMVLYK DKVYNIQIRY QKESQVYLLG TGLRGKEDFL SVSDIIDYFR KMPLLLIDGK NRGSRYQCTL THAAGYPLEH HHHHH

Attributs du produit: Recombinant Human LCP2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro533) of Human LCP2 fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,20 % glycerol, pH 8.5.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Autre désignation: Lymphocyte Cytosolic Protein 2/LCP2 (LCP2 Produits)

Synonymes: AI323664 Protein, BB161688 Protein, SLP-76 Protein, SLP76 Protein, twm Protein, Slp76 Protein, lymphocyte cytosolic protein 2 Protein, Lcp2 Protein, LCP2 Protein

Sujet: Lymphocyte cytosolic protein 2(LCP2)contains a SAM domain and a SH2 domain. It is highly expressed in spleen, thymus and peripheral blood leukocytes, T-cell and monocytic cell lines, but expressed at lower level in B-cell lines. LCP2 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. It is phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2 during post-translational modification. Studies using LCP2-deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.
Synonyms: Lymphocyte cytosolic protein 2,SH2 domain-containing leukocyte protein of 76 kDa,SLP-76 tyrosine phosphoprotein,SLP76,LCP2

Poids moléculaire: 62.6 kDa

UniProt: Q13094

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway