FCGR3A Protein (AA 17-208) (His tag)

N° du produit ABIN2181078

Détail du produit

Antigène: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 17-208

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR3A protéine est marqué à la His tag.

Marque: MABSol®

Séquence: AA 17-208

Attributs du produit: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Référence

Simonov, Ivanov, Smolov, Abbasova, Piskunov, Poteryaev: "Control of therapeutic IgG antibodies galactosylation during cultivation process and its impact on IgG1/FcγR interaction and ADCC activity." dans: Biologicals : journal of the International Association of Biological Standardization, Vol. 58, pp. 16-21, (2019) (PubMed).

Zhao, Li, Wu, Li, Liu, Wang, Liu, Li, Wang: "Identification of anti-CD16a single domain antibodies and their application in bispecific antibodies." dans: Cancer biology & therapy, pp. 1-9, (2019) (PubMed).

Détail du antigène

Antigène: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Autre désignation: Fc gamma RIIIA / CD16a (FCGR3A Produits)

Synonymes: CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein

Sujet: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Poids moléculaire: 23.7 kDa

NCBI Accession: NP_000560