FCGR3A Protein (His-Avi Tag,Biotin)

N° du produit ABIN7274282

Détail du produit

Antigène: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR3A protéine est marqué à la His-Avi Tag,Biotin.

Séquence: Gly17-Gln208(F176)

Pureté: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA. The affinity constant of 0.14μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Stock: 4 °C,-80 °C

Stockage commentaire: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Autre désignation: Fc gamma RIIIA (FCGR3A Produits)

Synonymes: CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein

Sujet: CD16FCRIIIA, Fc fragment of IgG, Fc gamma RIIIA, Fc-gamma RIII-alpha, FCGR3, FCGR3A, FCGRIII, FcgRIIIA, FcR-10, CD16A, FCG3, IGFR3, FcRIIIa, FcRIII, CD16, IMD20,Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Poids moléculaire: 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.