PCSK9 Protein (AA 31-692) (His tag,AVI tag,Biotin)

N° du produit ABIN2444171

Détail du produit

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 31-692

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette PCSK9 protéine est marqué à la His tag,AVI tag,Biotin.

Marque: MABSol®,PrecisionAvi

Séquence: AA 31-692

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 72 kDa. The protein migrates as 18 kDa and 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.

Pureté: >92 % as determined by reduced SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Référence

Wang, Amiss, Weidmann, Craik: "Structure-activity analysis of truncated albumin-binding domains suggests new lead constructs for potential therapeutic delivery." dans: The Journal of biological chemistry, Vol. 295, Issue 34, pp. 12143-12152, (2021) (PubMed).

Chen, Lichtor, Berliner, Chen, Liu: "Evolution of sequence-defined highly functionalized nucleic acid polymers." dans: Nature chemistry, Vol. 10, Issue 4, pp. 420-427, (2019) (PubMed).

Wang, Ghani, Bundock, Weidmann, Harvey, Edwards, Schroeder, Swedberg, Craik: "Calcium-Mediated Allostery of the EGF Fold." dans: ACS chemical biology, Vol. 13, Issue 6, pp. 1659-1667, (2019) (PubMed).

Détail du antigène

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Autre désignation: PCSK9 (PCSK9 Produits)

Synonymes: FH3 Protein, HCHOLA3 Protein, LDLCQ1 Protein, NARC-1 Protein, NARC1 Protein, PC9 Protein, AI415265 Protein, AI747682 Protein, Narc1 Protein, proprotein convertase subtilisin/kexin type 9 Protein, proprotein convertase subtilisin/kexin type 9 L homeolog Protein, PCSK9 Protein, pcsk9.L Protein, pcsk9 Protein, Pcsk9 Protein

Sujet: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Poids moléculaire: 13.8 kDa and 59.9 kDa