SOX2 Protein (TAT tag)

N° du produit ABIN2469109

Détail du produit

Antigène: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SOX2 protéine est marqué à la TAT tag.

Séquence: MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR

Pureté: < 95 % by SDS-PAGE gel and HPLC analyses.

niveau d'endotoxine: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Conseil sur la manipulation: As with any protein, exposing Sox2-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -20 °C

Stockage commentaire: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Sox2-TAT is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Date de péremption: 24 months

Détail du antigène

Antigène: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Autre désignation: Sox2 (SOX2 Produits)

Synonymes: ANOP3 Protein, MCOPS3 Protein, RGD1565646 Protein, Sox-2 Protein, lcc Protein, ysb Protein, cb236 Protein, wu:fb83g04 Protein, wu:fc14d07 Protein, zgc:65860 Protein, zgc:77389 Protein, SRY-box2 Protein, Xsox-2 Protein, anop3 Protein, mcops3 Protein, sox-2 Protein, SRY-box 2 Protein, SRY box 2 Protein, SRY (sex determining region Y)-box 2 Protein, SRY-box 2 S homeolog Protein, SOX2 Protein, Sox2 Protein, sox2 Protein, sox2.S Protein

Sujet: Sox2, also known as sex determining region Y (SRY)-box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, Myc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Sox2 and other transcription factors have been introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other nuclear proteins into primary as well as transformed cells. Recombinant human Sox2-TAT expressed in E. coli is a 36 kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue C-terminal TAT peptide (GGYGRKKRRQRRR).

ID gène: 6657

NCBI Accession: NP_003097

OMIM: 28195386

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis