WHSC1 Protein (DYKDDDDK Tag)

N° du produit ABIN2669701

Détail du produit

Antigène: WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus

Purification/Conjugué: Cette WHSC1 protéine est marqué à la DYKDDDDK Tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant MMSET / WHSC1 (accession number NP_579877.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 154 kDa.

Information d'application

Indications d'application: Recombinant MMSET / WHSC1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36me2 methyltransferase activity. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl Recombinant MMSET / WHSC1 protein at 2 hours at room temperature. Activity was detected by fluorography.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1))

Autre désignation: MMSET / WHSC1 (WHSC1 Produits)

Synonymes: WHSC1 Protein, fc12c04 Protein, wu:fc12c04 Protein, wu:fi20c01 Protein, si:rp71-77d7.2 Protein, whs Protein, nsd2 Protein, trx5 Protein, mmset Protein, reiibp Protein, MMSET Protein, NSD2 Protein, REIIBP Protein, TRX5 Protein, WHS Protein, RGD1565590 Protein, 5830445G22Rik Protein, 9430010A17Rik Protein, AW555663 Protein, C130020C13Rik Protein, D030027O06Rik Protein, D930023B08Rik Protein, Whsc1l Protein, mKIAA1090 Protein, nuclear receptor binding SET domain protein 2 Protein, Wolf-Hirschhorn syndrome candidate 1 Protein, NSD2 Protein, nsd2 Protein, WHSC1 Protein, Nsd2 Protein

Sujet: MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1, also known as NSD2, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15 % of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic μ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state.

Poids moléculaire: 154 kDa

Pathways: SARS-CoV-2 Protein Interactome