KDM3A Protein (DYKDDDDK Tag)

N° du produit ABIN2669704

Détail du produit

Antigène: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus

Purification/Conjugué: Cette KDM3A protéine est marqué à la DYKDDDDK Tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant JMJD1A / KDM3A (accession number NP_001140160.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 151.6 kDa.

Information d'application

Indications d'application: Recombinant JMJD1A / KDM3A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 60 nM Recombinant JMJD1A / KDM3A protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Autre désignation: JMJD1A / KDM3A (KDM3A Produits)

Synonymes: JHDM2A Protein, JHMD2A Protein, JMJD1 Protein, JMJD1A Protein, TSGA Protein, tsga Protein, jmjd1 Protein, jhdm2a Protein, jhmd2a Protein, jmjd1a Protein, 1700105C21Rik Protein, C230043E16Rik Protein, JHDM2a Protein, Jmjd1 Protein, Jmjd1a Protein, KDM2A Protein, TGSA Protein, Tsga Protein, mKIAA0742 Protein, KDM3A Protein, kdm3a Protein, lysine demethylase 3A Protein, lysine (K)-specific demethylase 3A Protein, lysine-specific demethylase 3A Protein, lysine (K)-specific demethylase 3A S homeolog Protein, KDM3A Protein, kdm3a Protein, Kdm3a Protein, LOC100453519 Protein, kdm3a.S Protein

Sujet: KDM3A (lysine (K)-specific demethylase 3A), also known as JMJD1A (Jumonji Domain Containing 1A) is a histone demethylase that preferentially demethylates mono- and dimethylated lysine 9 of histone H3, with a preference for the dimethylated residue. KDM3A has little or no activity on trimethylated lysine 9. KDM3A is involved in hormone-dependent transcriptional activation by participating in the recruitment to androgen-receptor target genes resulting in H3 lysine 9 demethylation and transcriptional activation. KDM3A is also involved in spermatogenesis where it regulates expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. KDM3A contributes to obesity resistance through its regulation of metabolic genes such as PPARα and UCP1.

Poids moléculaire: 151.6 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, L'effet Warburg