DOT1L Protein (AA 1-416) (GST tag)

N° du produit ABIN2669718

Détail du produit

Antigène: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-416

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette DOT1L protéine est marqué à la GST tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Recombinant DOT1L (1-416), an active truncation that includes amino acids 1-416 (accession number NP_115871.1) ,was expressed in E. coli cells and contains an N-terminal GST tag with an observed molecular weight of 72 kDa.

Information d'application

Indications d'application: Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K79 methyltransferase HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl DOT1L (1-416 aa) at 2 hours at room temperature. Activity was detected by fluorography.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Autre désignation: DOT1L (DOT1L Produits)

Synonymes: NV11307 Protein, DOT1L Protein, DOT1 Protein, KMT4 Protein, A630076O07 Protein, AW907654 Protein, Dot1 Protein, mDot1 Protein, histone-lysine N-methyltransferase, H3 lysine-79 specific Protein, DOT1 like histone lysine methyltransferase Protein, histone methyltransferase Protein, DOT1-like, histone H3 methyltransferase (S. cerevisiae) Protein, LOC100116648 Protein, DOT1L Protein, DOT1 Protein, Dot1l Protein

Sujet: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.

Poids moléculaire: 72 kDa