KDM1B Protein (full length) (GST tag)

N° du produit ABIN2669723

Détail du produit

Antigène: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Type de proteíne: Recombinant

Attributs du protein: full length

Origine: Humain

Source: Baculovirus

Purification/Conjugué: Cette KDM1B protéine est marqué à la GST tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Attributs du produit: Full-length Recombinant KDM1B / LSD2 (accession number XM_005248926.1) was expressed in was expressed in Sf9 cells and contains an N-terminal GST tag with an observed molecular weight of 116 kDa.

Information d'application

Indications d'application: Recombinant KDM1B / LSD2 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me2 and H3K4me1 demethylase. DMT Assay Conditions: 50 mM TrisCl, pH 8.0, 0.02 % Triton X-100, 10 nM FAD, 5 nM KDM1B / LSD2, 3.3 μM H3K4me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Autre désignation: KDM1B / LSD2 (KDM1B Produits)

Synonymes: AOF1 Protein, C6orf193 Protein, LSD2 Protein, bA204B7.3 Protein, dJ298J15.2 Protein, 4632428N09Rik Protein, AI482520 Protein, Aof1 Protein, lysine demethylase 1B Protein, lysine-specific histone demethylase 1B Protein, lysine (K)-specific demethylase 1B Protein, KDM1B Protein, LOC577549 Protein, kdm1b Protein, Kdm1b Protein

Sujet: KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (by similarity).

Poids moléculaire: 116 kDa

Pathways: L'effet Warburg