DFFA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2719434

Détail du produit

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette DFFA protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human DFFA / ICAD (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: Dffa,icad (DFFA Produits)

Synonymes: DFF-45 Protein, DFF1 Protein, ICAD Protein, A330085O09Rik Protein, DFF35 Protein, Dff45 Protein, ICAD-L Protein, ICAD-S Protein, DFFA Protein, zgc:92229 Protein, DNA fragmentation factor subunit alpha Protein, DNA fragmentation factor, alpha subunit Protein, DNA fragmentation factor, 45kDa, alpha polypeptide Protein, DNA fragmentation factor, alpha polypeptide Protein, DFFA Protein, Dffa Protein, dffa Protein

Sujet: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Poids moléculaire: 36.3 kDa

NCBI Accession: NP_004392

Pathways: Apoptose, Caspase Cascade in Apoptosis