EBI3 Protein (His tag)

N° du produit ABIN2719909

Détail du produit

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Purification/Conjugué: Cette EBI3 protéine est marqué à la His tag.

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human EBI3 / IL27B protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % , as determined by Coomassie stained SDS-PAGE.

niveau d'endotoxine: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 60 - 90 ng/ml, corresponding to a specific activity of 1.1 - 1.7 x 10^4 units/mg, determined by mouse splenocytes IL-2 inhibition induced by hIL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 20 mM NaHPO4, pH 6, 0.60 M NaCl.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Wang, Wei, Xiao, Liu, Zhang, Han, Chen, Hou, Ma, Shen, Li, Egwuagu, Wang: "A novel IL-23p19/Ebi3 (IL-39) cytokine mediates inflammation in Lupus-like mice." dans: European journal of immunology, Vol. 46, Issue 6, pp. 1343-50, (2016) (PubMed).

Détail du antigène

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Autre désignation: Ebi3,il27b (IL-27b Produits)

Synonymes: IL-27B Protein, IL27B Protein, EBI3 Protein, il-27rb Protein, si:dkey-97a13.4 Protein, EBI-3 Protein, IL-27 Protein, Epstein-Barr virus induced 3 Protein, Epstein-Barr virus induced gene 3 Protein, EBI3 Protein, ebi3 Protein, Ebi3 Protein

Classe de substances: Viral Protein

Sujet: This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.

Poids moléculaire: 50 kDa

NCBI Accession: NP_005746