ADD3 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- Antigène Voir toutes ADD3 Protéines
- ADD3 (Adducin 3 (Gamma) (ADD3))
- Type de proteíne
- Recombinant
- Attributs du protein
- Transcript Variant 1
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette ADD3 protéine est marqué à la Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Attributs du produit
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- Recombinant human Gamma-adducin (ADD3) (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Pureté
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ADD3 Protéine
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Commentaires
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Antigène
- ADD3 (Adducin 3 (Gamma) (ADD3))
- Autre désignation
- gamma-Adducin (Add3) (ADD3 Produits)
- Synonymes
- gamma-adducin Protein, add3 Protein, MGC89418 Protein, ADD3 Protein, DKFZp459E0630 Protein, ADDL Protein, AI463285 Protein, R75380 Protein, adducin 3 (gamma) Protein, adducin 3 Protein, adducin 3 L homeolog Protein, ADD3 Protein, add3 Protein, add3.L Protein, Add3 Protein
- Sujet
- Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
- Poids moléculaire
- 79 kDa
- NCBI Accession
- NP_058432
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