HLA-DQA1 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2722841

Détail du produit

Antigène: HLA-DQA1 (Major Histocompatibility Complex, Class II, DQ alpha 1 (HLA-DQA1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette HLA-DQA1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human HLA class II DQ alpha 1 / HLA-DQA1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: HLA-DQA1 (Major Histocompatibility Complex, Class II, DQ alpha 1 (HLA-DQA1))

Autre désignation: Hla Class II Dq alpha 1,hla-Dqa1 (HLA-DQA1 Produits)

Synonymes: CD Protein, CELIAC1 Protein, DQ-A1 Protein, GSE Protein, HLA-DQA Protein, BoLA-DQA Protein, hla-dqa1 Protein, major histocompatibility complex, class II, DQ alpha 1 Protein, major histocompatibility complex, class II, DQ alpha, type 1 Protein, major histocompatibility complex, class II, DQ alpha 1 L homeolog Protein, HLA-DQA1 Protein, BOLA-DQA1 Protein, hla-dqa1.L Protein

Sujet: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-ce [UniProtKB/Swiss-Prot Function]

Poids moléculaire: 27.8 kDa

NCBI Accession: NP_002113

Pathways: TCR Signaling, Cancer Immune Checkpoints