HLA-DRB4 Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2722842

Détail du produit

Antigène: HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette HLA-DRB4 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human HLA Class II DR beta 4 / HLA-DRB4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: HLA-DRB4 (Major Histocompatibility Complex, Class II, DR beta 4 (HLA-DRB4))

Autre désignation: Hla Class II Dr beta 4,hla-Drb4 (HLA-DRB4 Produits)

Synonymes: DR-4 Protein, DR4 Protein, DRB4 Protein, HLA-DR4B Protein, major histocompatibility complex, class II, DR beta 4 Protein, HLA-DRB4 Protein

Sujet: HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

Poids moléculaire: 27 kDa

NCBI Accession: NP_068818

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response