MMP1 Protein (Transcript Variant 1) (His tag)

N° du produit ABIN2726137

Détail du produit

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette MMP1 protéine est marqué à la His tag.

Application: Antibody Production (AbP), Standard (STD), Protein Interaction (PI), Functional Studies (Func)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human MMP-1 (transcript variant 1) protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 90 % , as determined by Coomassie stained SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg of protein as determine by the LAL method.

Biological Activity Comment: Human MMP-1 cleaves the peptide substrate Mca-KPLGL-Dpa-AR-NH2 with an activity above 200 pmol/min/μg.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Commentaires:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Autre désignation: Mmp-1 (MMP1 Produits)

Synonymes: CLG Protein, CLGN Protein, Mmp1a Protein, CG4859 Protein, Dm1-MMP Protein, Dmel\\CG4859 Protein, MMP-1 Protein, MMP1 Protein, Mmp 1 Protein, dMMP1 Protein, dm1-MMP Protein, dmmp1 Protein, l(2)k04809 Protein, mmp1 Protein, Mmp1 Protein, Mcol-A Protein, Mcola Protein, col4 Protein, mmp18 Protein, Clgn Protein, matrix metallopeptidase 1 Protein, Matrix metalloproteinase 1 Protein, matrix metalloproteinase 1 Protein, matrix metallopeptidase 1 (interstitial collagenase) Protein, matrix metallopeptidase 1a (interstitial collagenase) Protein, matrix metallopeptidase 1 S homeolog Protein, interstitial collagenase Protein, matrix metallopeptidase 8 L homeolog Protein, MMP1 Protein, Mmp1 Protein, RB11133 Protein, Mmp1a Protein, mmp1.S Protein, LOC100727966 Protein, mmp8.L Protein

Sujet: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Poids moléculaire: 54 kDa

NCBI Accession: NP_002412