NARF Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

N° du produit ABIN2726832

Détail du produit

Antigène: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 3

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette NARF protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human NARF (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Autre désignation: Narf (NARF Produits)

Synonymes: NARF Protein, IOP2 Protein, 4430402O11Rik Protein, RGD1310894 Protein, wu:fa03c01 Protein, zgc:92186 Protein, nuclear prelamin A recognition factor Protein, nuclear prelamin A recognition factor L homeolog Protein, NARF Protein, NAEGRDRAFT_78871 Protein, VDBG_04882 Protein, Tsp_07547 Protein, Tsp_07549 Protein, Narf Protein, narf Protein, narf.L Protein

Sujet: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Poids moléculaire: 44.5 kDa

NCBI Accession: NP_001033707