PICALM Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2728884

Détail du produit

Antigène: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette PICALM protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human PICALM (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Référence

Zhao, Sagare, Ma, Halliday, Kong, Kisler, Winkler, Ramanathan, Kanekiyo, Bu, Owens, Rege, Si, Ahuja, Zhu, Miller, Schneider, Maeda, Maeda, Sugawara, Ichida, Zlokovic: "Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance." dans: Nature neuroscience, Vol. 18, Issue 7, pp. 978-87, (2015) (PubMed).

Détail du antigène

Antigène: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Autre désignation: Picalm (PICALM Produits)

Synonymes: LAP-3 Protein, PEPS Protein, CALM Protein, CLTH Protein, LAP Protein, PAP180 Protein, fit-1 Protein, fit1 Protein, mKIAA4114 Protein, Calm Protein, leucine aminopeptidase 3 Protein, phosphatidylinositol binding clathrin assembly protein Protein, si:ch211-200p22.4 Protein, LAP3 Protein, PICALM Protein, Picalm Protein, si:ch211-200p22.4 Protein

Sujet: This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(1011)(p13q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 70.6 kDa

NCBI Accession: NP_009097

Pathways: Synaptic Membrane