SPAM1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2732494

Détail du produit

Antigène: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Type de proteíne: Recombinant

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette SPAM1 protéine est marqué à la Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Attributs du produit:

• Recombinant human SPAM1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Autre désignation: Spam1 (SPAM1 Produits)

Synonymes: AV039194 Protein, Ph-20 Protein, PH-20 Protein, PH20 Protein, HYA1 Protein, HYAL1 Protein, HYAL3 Protein, HYAL5 Protein, SPAG15 Protein, Spam Protein, XEH1 Protein, spam1 Protein, im:6902904 Protein, zgc:158668 Protein, sperm adhesion molecule 1 Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) Protein, Spam1 Protein, SPAM1 Protein, spam1.L Protein, spam1 Protein

Sujet: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1 another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 58.2 kDa

NCBI Accession: NP_003108