SPARC Protéine

N° du produit ABIN2732505

Détail du produit

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: CHO Cells

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human SPARC / Osteonectin protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5-0.7ug/mL.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: Sparc,osteonectin (SPARC Produits)

Synonymes: ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein

Sujet: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 43.7 kDa

NCBI Accession: NP_003109

Pathways: Autophagy