NAMPT Protéine

N° du produit ABIN2735308

Détail du produit

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human Visfatin / NAMPT protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED50 for this effect is 15.0-20.0 ng/ml.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Autre désignation: Visfatin,nampt (NAMPT Produits)

Synonymes: 1110035O14Rik Protein, PBEF Protein, PBEF1 Protein, VF Protein, VISFATIN Protein, AI314458 Protein, AI480535 Protein, NAmPRTase Protein, Pbef Protein, Pbef1 Protein, Visfatin Protein, visfatin Protein, pbef Protein, pbef1 Protein, nicotinamide phosphoribosyltransferase Protein, Nicotinamide phosphoribosyltransferase Protein, nicotinamide phosphoribosyltransferase S homeolog Protein, NAMPT Protein, Nampt Protein, nampt Protein, Smon_0680 Protein, Mrub_2845 Protein, Cseg_3704 Protein, BC1002_3997 Protein, Ftrac_2686 Protein, Varpa_5681 Protein, Celal_3900 Protein, Deima_0880 Protein, BC1001_3724 Protein, Celly_1696 Protein, Clole_1542 Protein, Tsp_05934 Protein, nampt.S Protein

Sujet: This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.

Poids moléculaire: 52.6 kDa

NCBI Accession: NP_005737