NMNAT3 Protein (AA 1-252) (His tag)

N° du produit ABIN2745774

Détail du produit

Antigène: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-252

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette NMNAT3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Réactivité croisée: Humain

Attributs du produit: Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.

Pureté: >95 % (SDS-PAGE)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NAD+ per min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 8.0, containing 300 mM sodium chloride, 1 mM DTT and 10 % glycerol.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Détail du antigène

Antigène: NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))

Autre désignation: NMNAT3 (NMNAT3 Produits)

Synonymes: PNAT-3 Protein, PNAT3 Protein, 4933408N02Rik Protein, nicotinamide nucleotide adenylyltransferase 3 Protein, si:ch73-170l17.1 Protein, Nmnat3 Protein, NMNAT3 Protein, si:ch73-170l17.1 Protein

Sujet: Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.

Poids moléculaire: ~27kDa (monomer)

UniProt: Q96T66